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May 27, 2025

Data-independent acquisition: Initial figures of merit

Data-independent acquisition (DIA) proteome profiling on the Orbitrap Astral

Data-independent (DIA) acquisition at IQ Proteomics, via Orbitrap Astral acquisition, is a complementary approach to our multiplexed workflows. DIA acquisition provides deep coverage, faster project completion, and improved pricing. An initial assessment of three cell lines showcases the following notable figures of merit:

• Coverage of >10,000 proteins

• >6 orders of magnitude dynamic range

• 5% median protein coefficient of variation

• Excellent biological response though robust quantitative data

We will provide additional insights on how both our DIA and multiplexed workflows at IQP provide unparalleled proteomic insights across a variety of applications. Please contact IQP with any questions or if interested in proceeding with these services.

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